Table 1.
Mass spectrometry analysis of several TTR variants subjected to H2O2 oxidation. Shown is the number of Met and Cys in the sequences, the increase in MW observed after H2O2 treatment with respect to native (non-oxidized) TTR and the number oxygen atoms equivalent to the observed MW change. The TTR variants differ only by the number of Met and Cys in the sequence.
| TTR variant | No. of Met | No. of Cys | increase in MW | Oxygen atoms |
|---|---|---|---|---|
| WT | 2 | 1 | 80 | 5 |
| V122I | 2 | 1 | 80 | 5 |
| C10A | 2 | 0 | 32 | 2 |
| M13I | 1 | 1 | 64 | 4 |
| T119M | 3 | 1 | 96 | 6 |
| F87M/L110M | 4 | 1 | 112 | 7 |
| rTTRa | 1 | 1 | 64 | 4 |
| M13I rTTR | 0 | 1 | 48 | 3 |
| C10A rTTR | 1 | 0 | 16 | 1 |
a
rTTR is recombinant protein lacking Met at position −1 (34)