Table 2.
Midpoint transition values (Cm) of TTR denaturation/renaturation curves in urea. All Cm values are presented in molar units. TTR unfolding and refolding was measured by tryptophan fluorescence; tetramer disassembly and reassembly was measured by resveratrol binding fluorescence.
| TTR variant | Unfolding | Refolding | Disassembly | Reassembly | ||||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| Cm | p | Cm | p | Cm | p | Cm | p | |
| WT TTR | 3.5 ±0.06 | 3.5 ±0.12 | 3.4 ±0.09 | 3.4 ± 0.08 | ||||
| oxi-WT | 3.0 ±0.05 | **** | 2.8 ±0.12 | **** | 2.8 ±0.19 | ** | 2.8 ± 0.03 | *** |
| car-WT | 3.4 ±0.15 | ns | 3.3 ±0.05 | ** | 2.8 ±0.27 | ** | 3.1 ±0.00 | ** |
|
| ||||||||
| V122I TTR | 3.4 ±0.06 | 3.4 ±0.07 | 2.8 ±0.07 | 2.9 ±0.08 | ||||
| oxi-V122I | 2.8 ±0.04 | **** | 2.8 ±0.05 | **** | 2.4 ±0.09 | **** | 2.5 ±0.05 | **** |
| car-V122I | 3.4 ±0.03 | ns | 3.3 ±0.06 | ns | 2.5 ±0.04 | *** | 2.5 ±0.02 | **** |
p values measure significant changes in Cm for the oxidized proteins with respect to their non-oxidized counterparts. Unpaired T-test: ns (no significant), p > 0.05;
**
p ≤0.01;
***
p ≤0.001;
****
p ≤0.0001.