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. 2013 Mar 20;8(3):e58697. doi: 10.1371/journal.pone.0058697

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© 2013 You et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, schematic representation of Mst1 deletion mutants. B, flag-GAPDH expression vector in combination of either empty vector or expression vectors of Myc-Mst1 mutants were cotransfected into HEK293T cells. Extracted proteins were precipitated by anti-Myc antibody and then separated by 12% SDS-PAGE. The transferred membrane was immunoblotted with either HRP-conjugated anti-FLAG or HRP-conjugated anti-Myc antibody. Lysates were also immunoblotted with anti-Flag antibody to show the expression levels of Flag-GAPDH in HEK293T cells. C, schematic representation of GAPDH deletion mutants. D, Flag-Mst1 expression vector in combination of either empty vector or expression vectors of Myc-GAPDH mutants were cotransfected into HEK293T cells. Extracted proteins were precipitated by anti-Myc antibody and then separated by 12% SDS-PAGE. The transferred membrane was immunoblotted with either HRP-conjugated anti-FLAG or HRP-conjugated anti-Myc antibody. Lysates were also immunoblotted with anti-Flag antibody to show the expression of Flag-Mst1 in HEK293 cells.