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. 2013 Mar 20;8(3):e58697. doi: 10.1371/journal.pone.0058697

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© 2013 You et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, 0.1 µg active Mst1 was incubated with different amounts of recombinant GAPDH for 60 min in the presence of ³²P-ATP in an in vitro phosphorylation assay. B, 0.1 µg active Mst1 was incubated with 4 µg bacterially expressed recombinant GAPDH for different time points in the presence of ³²P-ATP in an in vitro phosphorylation assay. Phosphorylation was detected by autoradiography. The data are representatives of 4 independent experiments.