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. 2013 Mar 20;8(3):e58697. doi: 10.1371/journal.pone.0058697

Figure 7. Overexpression of GAPDH enhances Mst1 mediated cardiomyocyte apoptosis.

Figure 7

A, NRVMs were transduced with either Ad-LacZ or Ad-GAPDH (MOI = 30). 48 hr after transduction, cell lysates were subjected to western blot analysis to detect the expression of GAPDH. B, NRVMs were transduced with Ad-LacZ or Ad-GAPDH at 30 mois. Twenty-four hours after transduction, myocytes were transduced with Ad-LacZ or Ad-Mst1 at 30 mois. 48 hours after the second transduction, cytoplasmic accumulation of mono- and oligonucleosomes was quantitated by the Cell Death Detection ELISA. Values are means ± SEM obtained from 4 experiments. C, NRVMs were transduced with Ad-LacZ, Ad-GAPDH, or Ad-DNMST with different combinations (total 60 mois). 48 hours after the transduction, the cells were treated with chelerythrine (5 µM) for 2 hours, the cytoplasmic accumulation of mono- and oligonucleosomes was then quantitated by the Cell Death Detection ELISA. Values are means ± SEM obtained from 4 experiments.