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. 2013 Mar 20;8(3):e58697. doi: 10.1371/journal.pone.0058697

Figure 9. Knockdown of GAPDH attenuates Mst1 activation and cell apoptosis in response to hypoxia/reoxygenation.

Figure 9

A, NRVMs were transfected with either control siRNA or GAPDH siRNA. 72 hours after transfection, cells were treated with hypoxia for 12 hours and reoxygenation for 24 hours. Mst1 was then immunoprecipitated and its activity was determined by an in vitro kinase assay using histone H2B as a substrate. B, NRVMs were transfected with either control siRNA or GAPDH siRNA. 72 hours after transfection, cells were treated with hypoxia/reoxygenation and the cell apoptosis was determined by using the TUNEL staining kit (Roche). Values are means ± SEM obtained from 4 experiments.