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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Exp Gerontol. 2012 Dec 9;48(10):1006–1013. doi: 10.1016/j.exger.2012.12.001

Figure 1. Diagram depicting the chronological lifespan to replicative lifespan assay.

Figure 1

Cells are aged chronologically in synthetic complete (SC) medium with either 2% glucose (control) or 0.05% glucose (DR). At different age points, an aliquot is removed from the chronologically aging culture and spotted onto rich medium (YPD) for replicative lifespan analysis. Individual cells are arrayed on the YPD plate and those replicative lifespan is determined.