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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Alzheimers Dement. 2013 Jan 30;9(2):99–112. doi: 10.1016/j.jalz.2012.11.005

Figure 4. The o-ELISAs specifically detect Aβ species in AD TBS brain extracts.

Figure 4

(A–B) AD-TBS was serially diluted and assayed with the two o-ELISAs (A) and the conventional Aβ1-x ELISA (B). All three ELISAs showed highly linear concentration curves. (C) After two sequential pre-clearing steps, AD-TBS was subjected to IP by antibodies 3D6 or 3F3 or NAB61. As numbered, the various supernatants were assayed by o-ELISAs (bar graph) and the corresponding immunoprecipitates by WB. (WB: 6E10+2G3+21F12).