Table 1.
Validation data: Calibration, intra- and inter-day precision and accuracy of method at three concentrations (L=50 nM, M=500 nM and H=1000 nM).
| Method Validation | 3-oxo-C12-HSL | C12-TA |
|---|---|---|
| Calibration range (nM) | 5–1000 | 5–1000 |
| Linearity (r2) | 0.9999 | 0.9937 |
| LLOQ (nM) | 10 | 10 |
| Concentration (nM) | Low | Medium | High | Low | Medium | High |
|---|---|---|---|---|---|---|
| 50 | 500 | 1000 | 50 | 500 | 1000 | |
| Intra-day (n=3) | ||||||
| Precision | 5.15 | 0.98 | 0.96 | 20.33 | 4.57 | 6.82 |
| Accuracy | 85.27 | 101.12 | 98.91 | 104.29 | 101.86 | 100.08 |
| Inter-day (n=3) | ||||||
| Precision | 3.28 | 3.11 | 0.7 | 3.12 | 12.47 | 4.09 |
| Accuracy | 102.33 | 99.23 | 100.28 | 103.4 | 91.37 | 101.94 |