Figure 1. Study design.

We sorted the human tumor cells of a squamous cell lung cancer xenograft into CD133+ and CD133− subpopulations. We then performed CNA analysis using genotyping arrays on peripheral blood mononuclear cells (PBMC) as well as both the CD133+ and CD133− subpopulations. We compared the genomes of the CD133+ population and PBMC sample to identify somatic mutations. Finally, we performed whole transcriptome sequencing (RNA-seq) of the CD133+ and CD133− subpopulations to evaluate their mRNA isoform expression differences and similarities.