Table 1. The relative expression of PXR, CYP3A4 and hsa-miR-148a in human liver samples.
Sample No. | PXR mRNA | PXR protein | CYP3A4 mRNA | CYP3A4 protein | miR-148a |
1 | 0.021 | 0.104 | 3.426 | 2.505 | 1.456 |
2 | 0.090 | 0.672 | 17.202 | 11.329 | 0.423 |
3 | 0.049 | 0.454 | 2.492 | 3.750 | 0.530 |
4 | 0.107 | 0.319 | 2.304 | 5.177 | 0.401 |
5 | 0.059 | 0.451 | 4.212 | 5.516 | 0.359 |
6 | 0.115 | 0.486 | 7.123 | 14.306 | 0.455 |
7 | 0.052 | 0.386 | 4.274 | 10.836 | 0.453 |
8 | 0.023 | 0.279 | 0.266 | 1.000 | 0.550 |
9 | 0.077 | 0.893 | 8.498 | 16.002 | 0.285 |
10 | 0.053 | 0.198 | 7.177 | 7.928 | 0.406 |
11 | 0.056 | 0.250 | 2.764 | 2.329 | 0.665 |
12 | 0.053 | 0.345 | 7.856 | 16.663 | 0.497 |
13 | 0.143 | 0.771 | 15.766 | 19.160 | 0.467 |
14 | 0.041 | 0.393 | 4.652 | 6.198 | 0.123 |
15 | 0.111 | 0.456 | 9.540 | 8.236 | 0.701 |
16 | 0.033 | 0.276 | 1.335 | 0.958 | 0.627 |
17 | 0.167 | 0.732 | 8.721 | 14.018 | 0.620 |
18 | 0.052 | 0.297 | 5.657 | 6.700 | 0.559 |
19 | 0.062 | 0.214 | 9.563 | 10.117 | 0.726 |
20 | 0.076 | 0.396 | 4.290 | 4.353 | 1.838 |
21 | 0.118 | 0.841 | 10.731 | 9.453 | 1.735 |
22 | 0.055 | 0.578 | 8.798 | 12.492 | 1.590 |
23 | 0.045 | 0.562 | 11.558 | 13.107 | 0.778 |
24 | 0.033 | 0.681 | 1.402 | 1.706 | 1.738 |
The relative RNA levels of mRNAs and miRNA were measured using qPCR with the normalization to the GAPDH mRNA level and the U6 snRNA level, respectively. The relative protein levels were measured using immunoblot with the normalization to the GAPDH protein level.