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. 2013 Mar 20;8(3):e59667. doi: 10.1371/journal.pone.0059667

Figure 4. Effect of miR-181a inhibitor on mouse granulosa cell (mGC) proliferation.

Figure 4

mGC was transfected with indicated miR-181a inhibitor (anti-sense oligonucleotide of miR-181a) or miRNA inhibitor negative control (miRNA inhibitor control) for 48 h. (A) MiR-181a expression was measured by qRT-PCR. (B) The proliferation of mGC was examined by CCK-8 after transfection of miR-181a inhibitor. Cyclin D2 mRNA (C) and protein (D) levels measured by qRT-PCR and Western blotting. (E) qRT-PCR and (F) Western blot analysis showed acvr2a mRNA and protein levels in mGC treated with miR-181a inhibitor. Relative protein levels were measured by densitometry using Quantity One Software and normalized to β-actin, the control group; the ratios were presented above the Western blot bands. *p<0.05, **p<0.01, compared with controls.