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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Biochem Pharmacol. 2013 Jan 31;85(8):1171–1181. doi: 10.1016/j.bcp.2013.01.021

Figure 2.

Figure 2

A. Absorbance spectra of 10 µM MRS5704 in solvents of different polarity (DMSO, methanol, Tris buffer, PBS). All spectra were recorded using a SpectraMax M5 microplate reader with a cuvette (solution) or 96-well black plates (membranes). MRS5704 in DMSO solution has three maxima in the absorbance spectra at 282, 372 and 398 nm. However, in more polar buffers like methanol or PBS, the absorption is lower and slightly red-shifted with maxima at 285, 384 and 410 nm. B. Fluorescence spectra of 10 µM MRS5704 in DMSO and PBS using 384 nm excitation wavelength. MRS5704 shows maximum at 508 nm in PBS and at 437 and 455 nm in DMSO. It is noteworthy that the fluorescence in a more polar solvent (PBS) not only shifted to longer wavelength, but the amplitude was significantly decreased.