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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Biochem Pharmacol. 2013 Jan 31;85(8):1171–1181. doi: 10.1016/j.bcp.2013.01.021

Figure 3.

Figure 3

Fluorescence micrograph of 70 nM MRS5218 bound to hA3AR expressed by CHO cells following incubation for the indicated times: A) 5 min; B) 15 min; C) 30 min; D) 60 min; E) 90 min; F) 120 min. hA3AR-expressing CHO cells in the absence of any fluorescence ligand were used as controls, but cell fluorescence was not observed. The images were acquired with Zeiss Axioimager D1 equipped with a filter set 50 (Zeiss) with excitation and emission wavelengths at BP640/30 nm and BP690/50 nm, respectively.