Skip to main content
. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Biochem Pharmacol. 2013 Jan 31;85(8):1171–1181. doi: 10.1016/j.bcp.2013.01.021

Figure 8.

Figure 8

Figure 8

A) Saturation binding in FCM assays using HEK293 cells expressing the mA3AR. The data are plotted as MESF values versus the concentration of MRS5218. B) Association and C) dissociation kinetics of MRS5218 binding to the mA3AR expressed in HEK293 cells by FCM. For the saturation assays, the cells were incubated with increasing concentrations of MRS5218 for 2 h prior to measurement of fluorescence. In the kinetic binding assays, MRS5218 was used at a concentration of 5 nM. Cells were incubated with MRS5218 for 2 h in the dissociation studies after which fluorescence was measured at the times indicated following the addition of NECA (100 µM). Results of a representative experiment performed in triplicate are shown. Average binding parameters (mean±SEM) from three experiments are summarized in the figures.