Table 3.
Results of culture, ELISA, ZN, IS900 PCR and qPCR in PB tissues
| Sr.No | Animal Id | Pathological Type | Culture | ELISA (SP) | ZN | IS900 PCR | qPCR | |
|---|---|---|---|---|---|---|---|---|
| Int. | MLN | |||||||
| 1 | AV24 | Paucibacillary | + | 3.29 | + | - | 1.582 × 104 | 1.073 × 105 |
| 2 | AV27 | Paucibacillary | + | 2.71 | + | - | 1.775 × 104 | 1.046 × 104 |
| 3 | AV30 | Paucibacillary | - | 1.04 | + | - | 6.141 × 103 | 1.565 × 103 |
| 4 | AV33 | Paucibacillary | + | 3.715 | + | - | 1.296 × 104 | 6.637 × 103 |
| 5 | AV35 | Paucibacillary | - | 86.11 | + | - | 8.441 × 103 | 4.209 × 103 |
| 6 | AV37 | Paucibacillary | + | 136.33 | + | - | 9.991 × 103 | 3.404 × 103 |
| 7 | AV38 | Paucibacillary | - | 18.75 | - | - | 3.369 × 103 | 1.734 × 103 |
| 8 | AV39 | Paucibacillary | - | 26.17 | - | + | 3.501 × 103 | 2.904 × 103 |
| 9 | AV41 | Paucibacillary | - | NT | + | - | 1.292 × 103 | 8.279 × 102 |
| 10 | AV44 | Paucibacillary | - | NT | + | - | 1.123 × 103 | 9.158 × 102 |
| 11 | AV46 | Paucibacillary | - | NT | + | + | 9.083 × 102 | 8.154 × 102 |
| 12 | AV48 | Paucibacillary | + | NT | + | - | 1.054 × 103 | 1.369 × 103 |
| 13 | AV49 | Paucibacillary | - | NT | + | - | 1.927 × 104 | 7.95 × 103 |
| 14 | AV50 | Paucibacillary | - | NT | + | - | 1.238 × 103 | 8.640 × 103 |
| 15 | AV51 | Paucibacillary | - | NT | + | - | 4.935 × 103 | 3.08 × 103 |
| 16 | AV67 | Uninfected control | - | 5.312 | - | - | - | - |
| 17 | AV68 | Uninfected control | - | −21.23 | - | - | - | - |
| 18 | AV69 | Uninfected control | - | 1.112 | - | - | - | - |
| 19 | AV70 | Uninfected control | - | 13.653 | - | - | - | - |
| 20 | AV71 | Uninfected control | - | −23.114 | - | - | - | - |
| 21 | AV72 | Uninfected control | - | −34.52 | - | - | - | - |
Int = Intestine, MLN = Mesenteric lymph node, NT = Not tested, + = Positive, - = Negative, SP > 70 is positive.