Figure 4.

Fluorescent microscopy of GFAP-labeling in the ipsilateral and contralateral hemispheres. At 1-day post-FPI, only minimal GFAP-labeling is observed in the contralateral (A) and ipsilateral (B) hemispheres. At 3 days post-FPI, only minimal GFAP-labeling is observed in the contralateral hemisphere (C), but a robust number of GFAP-labeled astrocytes are observed in the ipsilateral hemisphere (D). This pattern of labeling is also evident at 7 days post-FPI (E,F). It is pertinent to note that in the contralateral hemisphere (E), an overall depletion of GFAP-labeling is observed. This is similar to the observation in Figure 3 (E,F), in which the contralateral hemisphere corresponding to the lesion site also appeared depleted of GFAP-labeling. At 30 days post-FPI (G,H), GFAP-labeled astrocytes are widely distributed throughout both, ipsilateral (G) and contralateral (H) hemispheres. Note, that we have also provided light microscopic images of DAB-reacted tissue (Figure 5) demonstrating these same observations. Scale bar in (A) = 50 μm for all images.