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. Author manuscript; available in PMC: 2013 Mar 21.
Published in final edited form as: Dev Cell. 2011 Aug 25;21(3):559–574. doi: 10.1016/j.devcel.2011.07.014

Figure 2.

Figure 2

Wnt4 is directly activated by Wt1 in kidney mesenchyme in vivo and in vitro.

A. Wt1 and Wnt4 mRNA in control and Wt1 knockdown M15 cells.

B. Wnt4 mRNA expression in FACS sorted control (Wt1co/GFP) and Wt1-deficient (Nes-Cre Wt1co/GFP) e12.5 kidney mesenchyme cells.

C. ChIP of putative WREs using Wt1 antibodies in control and Wt1 knockdown M15 cells.

D. ChIP of putative WREs using Wt1 antibodies in FACS sorted control (Wt1co/GFP) and Wt1-deficient (Nes-Cre Wt1co/GFP) e12.5 kidney mesenchyme cells.

E. Luciferase reporter assays of wild type and mutant putative WREs in M15 cells.