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Wnt4 is directly repressed by Wt1 in epicardium in vivo and in vitro.
A. mRNA of Wt1 and Wnt4 expression in control and Wt1 knockdown MEEC (epicardium) cells.
B. mRNA of Wt1 and Wnt4 expression in FACS sorted control (Wt1co/GFP) and Wt1-deficient (Gata5-Cre Wt1co/GFP) epicardium.
C. ChIP with Wt1 antibodies of putative WREs in control and Wt1 knockdown MEEC cells.
D. Luciferase reporter assays of wild type and mutant putative WREs in MEEC cells.
