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. Author manuscript; available in PMC: 2013 Mar 21.
Published in final edited form as: Dev Cell. 2011 Aug 25;21(3):559–574. doi: 10.1016/j.devcel.2011.07.014

Figure 4.

Figure 4

In vitro and in vivo co-factors regulating Wnt4 expression in a tissue-specific manner downstream of Wt1.

A. ChIP using p300 antibodies in FACS sorted control (Wt1co/GFP) and Wt1-deficient (Nes-Cre Wt1co/GFP) e12.5 kidney mesenchyme cells.

B. ChIP using Cbp antibodies in FACS sorted control (Wt1co/GFP) and Wt1-deficient (Nes-Cre Wt1co/GFP) e12.5 kidney mesenchyme cells.

C-E. ChIP with p300 (C), Cbp (D) and Wt1 (E) antibodies in control (lacZ), Wt1 and a combined Cbp-p300 knockdown M15 cells.

F. mRNA of indicated genes in control, Cbp, p300 and Cbp/p300 knockdown M15 cells.

G. ChIP with Wt1 or Basp1 antibodies of WREs in control, Wt1 and Basp1 knockdown MEEC cells.

H. mRNA of indicated genes in control and Basp1 knockdown cells.

I. mRNA of indicated genes in control, Basp1 and Cbp/p300 knockdown M15 (kidney) and MEEC (epicardial) cells.