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. Author manuscript; available in PMC: 2013 Mar 21.
Published in final edited form as: Dev Cell. 2011 Aug 25;21(3):559–574. doi: 10.1016/j.devcel.2011.07.014

Figure 7.

Figure 7

CTCF and cohesin are essential for insulation but are dispensable for Wnt4 expression and Wt1-mediated chromatin flip-flop.

A. A graphic illustration of the complete Wnt4 locus and neighbouring genes as demarcated by U1 and D1.

B. ChIP with CTCF, Sa2 and Rad21 antibodies of control, Ctcf, Sa2 and Rad21 M15 cells at U1 (left panel) and D1 (right panel) sites.

C. ChIP with CTCF, Sa2 and Rad21 antibodies of control, Ctcf, Sa2 and Rad21 MEEC cells at U1 (left panel) and D1 (right panel) sites.

D. ChIP using H3K27Me3 (top), H3K4Me3 (middle) and H3Ac (bottom) antibodies in control, Ctcf, Sa2 and Rad21 knockdown M15 cells.

E. ChIP using H3K27Me3 (top), H3K4Me3 (middle) and H3Ac (bottom) antibodies in control, Ctcf, Sa2 and Rad21 knockdown MEEC cells.

F. mRNA of indicated genes in control, Wt1, Ctcf, Sa2 and Rad21 knockdown M15 cells.

G. mRNA of indicated genes in control, Wt1, Ctcf, Sa2 and Rad21 knockdown MEEC cells.

H. mRNA of indicated genes in Wt1-deficient kidney mesenchyme and epicardial cells in vivo normalized to control cells.