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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Cell Physiol. 2013 Apr;228(4):781–790. doi: 10.1002/jcp.24226

Figure 2. Inhibition of NF-kappa B by Vpr involves IκBα regulation but not PARP-1/GR signaling.

Figure 2

Macrophages from donor one (A) and TMZ-BL cells (B) were traduced with 5 moi of AdNull or AdVpr and 5moi of AdNF-κB luciferase reporter construct and after four hours with media or RU486 (1 uM) for one day. The cells were then treated for one additional day with TNF-α (10 ng/mL) and/or RU486 (1 uM). P values: P= 0.024, P= 0.55, P= 0.056, P= 0.57 (A) and P= 0.012, P= 0.005, P= 0.001, P= 5.86×10−4 (B) respectively. Effect of Vpr on IκBα phosphorylation and degradation in response to TNF-α (10 ng/mL) treatment in macropages (C) and PMA differentiated U937 cells. D. Effect of Vpr on PARP-1 and NF-κB translocation nuclear translocation in response to thirty minutes of TNF-α (10 ng/mL) treatment in PMA differentiated U937 cells. (* P≤ .05, ** P≤ .001, ns: non-significant)