Figure 3. Toxicity of Vpr and generation of knockdown U1/U937 cells.
A. MTT values after 2 and 3 days of culture for U87-MG cells, macrophages, and PMA differentiated U937 cells that were transduceded with 5 moi of AdNull or AdVpr. (P= 0.004, P= 0.001, P= 0.049, P= 0.027, P= 0.16, P= 0.43). B–C. Levels of HSP27 and VprBP in AdNull and AdVpr transduced or AdVpr PMA differentiated U937 stable cell lines expressing shNull, shHSP27 #2 and shVprBP #2 shRNA.