Table 4.
Thermodynamic Parameters for Binding of LSD1 to CoREST286–482 at Different Temperatures d
| T (°C) | Kd (nM) | ΔH (kcal mol−1) | TΔS (kcal mol−1) | ΔG (kcal mol−1) | Stoichiometry |
|---|---|---|---|---|---|
| 15 | 21.7 ± 10.9 | −4.24 ± 0.17 | 5.86 ± 2.95 | −10.1 ± 5.07 | 0.99 ± 0.02 |
| 25 | 3.18 ± 2.54 | −12.1 ± 0.39 | −0.49 ± 0.39 | −11.6 ± 9.27 | 1.01 ± 0.02 |
| 30 | 3.70 ± 1.74 | −16.1± 0.27 | −4.42 ± 2.08 | −11.7 ± 5.50 | 1.03 ± 0.01 |
| 35 | 8.03 ± 4.10 | −20.2 ± 0.57 | −8.79 ± 4.49 | −11.4 ± 5.82 | 0.97 ± 0.02 |
50 mM sodium phosphate and 1 mM DTT, pH 7.4