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. Author manuscript; available in PMC: 2013 Mar 21.
Published in final edited form as: Biochemistry. 2010 Dec 30;50(4):546–557. doi: 10.1021/bi101776t

Table 5.

Thermodynamic Parameters for Binding of LSD1 to CoREST286–482 at Different pH (25 °C) e

pH Kd (nM) ΔH (kcal mol−1) TΔS (kcal mol−1) ΔG (kcal mol−1) Stoichiometry
6.0 4.18 ± 0.96 −8.56 ± 0.01 2.87 ± 0.66 −11.4 ± 2.62 0.90 ± 0.004
7.0 6.75 ± 1.49 −12.9 ± 0.15 −1.54 ± 0.34 −11.4 ± 2.51 1.00 ± 0.01
8.0 7.66 ± 2.91 −15.6 ± 0.32 −4.48 ± 1.71 −11.1 ± 4.22 1.00 ± 0.01
9.0 nb nb nb nb nb
e

50 mM sodium phosphate, 50 mM glycine, and 1 mM DTT, pH 6.0, 50 mM sodium phosphate, 50 mM glycine, and 1 mM DTT, pH 7.0, 40 mM sodium phosphate, 30 mM glycine, and 1 mM DTT, pH 8.0, 40 mM sodium phosphate, 30 mM glycine, and 1 mM DTT, pH 9.0. nb = no binding curve observed