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. 2013 Feb 15;32(6):805–815. doi: 10.1038/emboj.2013.24

Figure 4.

Figure 4

Suppression of uls1-Δ by rap1 alleles. (A) Schematic representation of S. cerevisiae Rap1. The sequence surrounding lysine 246 is indicated. (B) Suppression of uls1-Δ by rap1-Δ2-309. Strains 196-11c and 196-13b (WT), 196-5a and 196-6a (uls1-Δ), 198-16a and 198-19a (uls1-Δ rap1-Δ2-228) and 196-1a and 196-7c (uls1-Δ rap1-Δ2-309) were grown to stationary phase. Telomere fusions were amplified with 30 and 34 cycles. (C) Suppression of uls1-Δ by mutation of Rap1 lysine 246. rap1-1R is rap1-K246R and rap1-2R is rap1-K240R, K246R. Strains 210-2d and 210-3d (WT), 210-5b and 210-4c (uls1-Δ), 210-10b and 210-5d (uls1-Δ rap1-1R), 210-1b and 210-7b (rap1-1R), 212-2d and 212-10b (WT), 212-1a and 212-8a (uls1-Δ), 212-5b and 212-17d (uls1-Δ rap1-2R), 212-3b and 212-4c (rap1-2R) were grown to stationary phase. Telomere fusions were amplified with 30 and 34 cycles.