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. 2013 Feb 19;32(6):816–828. doi: 10.1038/emboj.2013.28

Figure 4.

Figure 4

IKKα is required for IRF3-dependent functional maturation of DC. (A) BMDC from WT and IkkαAA/AA mice were stimulated with LPS and cytokine production measured in culture supernatants by ELISA at the indicated time points. Data are represented as mean±s.e.m. of n=4, statistical analysis was performed with Student’s t-test; ***P≤0.001. (B) BMDC from WT, IRF3, IRF3+IRF7 knockout (Irf3−/−; Irf3/7−/−) and IkkαAA/AA mice were loaded with MHC II-restricted OVA peptide (OVA323–339; OVAp) with and without LPS stimulation for 24 h before co-culture with naïve CD4+ OT-II T cells. IFNγ was measured in culture supernatants after 72 h. (C) WT, Irf3−/− and Irf3/7−/− BMDC were stimulated with LPS and cytokine production measured in culture supernatants by ELISA at the indicated time points. Data are represented as mean±s.e.m. of n=3.