Figure 6.

IFNβ-promoter activity is IKKα dependent. (A) WT, Ikkα^−/− and Ikkβ^−/− MEF cells were transfected with 1 μg/ml dsRNA (p(I:C)) and IFNβ mRNA expression measured at the indicated time points by qRT–PCR, data are expressed as fold induction over control normalized to CPH expression. (B) WT, Ikkα^−/−, Ikkβ^−/−, Rela^−/− and Irf3^−/− MEF cells were co-transfected with an IFNβ-promoter reporter vector expressing firefly luciferase (IFNβ-Luc; FL) and a constitutive renilla-luciferase reporter (RL); IFNβ-promoter activity was measured by dual luciferase assay 6 h after p(I:C) stimulation, data are expressed as fold induction of FL activity normalized to RL (FL/RL). Experiments were performed in triplicate and data represented as mean±s.e.m. of three independent experiments. (C) IB analysis of IRF3 Ser396 phosphorylation (pIRF3) in WT and Ikkα^−/− MEF cells after p(I:C) stimulation at the indicated time points. Representative data from three independent experiments are shown.

Source data for this figure is available on the online supplementary information page.

Source Data for Figure 6 ^{(324.8KB, pdf)}