Figure 6.
IFNβ-promoter activity is IKKα dependent. (A) WT, Ikkα−/− and Ikkβ−/− MEF cells were transfected with 1 μg/ml dsRNA (p(I:C)) and IFNβ mRNA expression measured at the indicated time points by qRT–PCR, data are expressed as fold induction over control normalized to CPH expression. (B) WT, Ikkα−/−, Ikkβ−/−, Rela−/− and Irf3−/− MEF cells were co-transfected with an IFNβ-promoter reporter vector expressing firefly luciferase (IFNβ-Luc; FL) and a constitutive renilla-luciferase reporter (RL); IFNβ-promoter activity was measured by dual luciferase assay 6 h after p(I:C) stimulation, data are expressed as fold induction of FL activity normalized to RL (FL/RL). Experiments were performed in triplicate and data represented as mean±s.e.m. of three independent experiments. (C) IB analysis of IRF3 Ser396 phosphorylation (pIRF3) in WT and Ikkα−/− MEF cells after p(I:C) stimulation at the indicated time points. Representative data from three independent experiments are shown.
Source data for this figure is available on the online supplementary information page.