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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: Dev Biol. 2012 Nov 27;375(2):105–116. doi: 10.1016/j.ydbio.2012.11.018

Fig. 3.

Fig. 3

Ureteric bud branching is not altered at early stages in Mi2βΔ/Δ kidneys. (A–D) Immunostaining of the UB with cytokeratin. At E12.5 and E13.5 UB branching was similar in wild type (A, C) and Mi2βΔ/Δ (B, D) kidneys. (E and F) Whole mount in situ hybridization of Ret in E15.5 wild type (E) and Mi2βΔ/Δ (F) kidneys. Ret expression at UB tips is preserved in Mi2βΔ/Δ kidneys. (G) Quantification of UB branching as measured by tip counting of sections from E12.5–E15.5 wild type (black bars) and Mi2βΔ/Δ (white bars) kidneys. At E12.5 and E13.5, there was no significant difference (p = 0.27) in the average number of UB tips per section in wild type (4 ± 0.45 and 6 ± 0.87) and Mi2βΔ/Δ (4 ± 0.55 and 6 ± 0.71). A significant reduction (p < 0.001) in the average number of UB tips per section was observed in Mi2βΔ/Δ kidneys compared to wild type kidneys at E14.5 (8 ± 2.12 vs. 11 ± 2.12) and E15.5 (12 ± 1.67 vs. 18 ± 1.48).