FIG 1.
Schematic of plasmid vectors used for stable episomal transfection of E. histolytica clone A1 trophozoites. Neomycin phosphotransferase (neoR) flanked by the 5′- and 3′-untranslated sequence of an E. histolytica actin gene was used as a selectable marker. Ehcp-a1, -a2, and -a5 are flanked by the respective gene-specific 3′- and 5′-untranslated regions (11). For all other plasmids, the coding sequences of the ehcp genes are flanked by the 5′-untranslated sequence of an E. histolytica lectin gene and by a 3′-untranslated sequence of an E. histolytica actin gene. MCS, multiple cloning site.