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. 2013 Mar 26;4(2):e00072-13. doi: 10.1128/mBio.00072-13

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Copyright © 2013 Matthiesen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 2 — Substrate gel electrophoresis of E. histolytica clone B2, clone A1, and transgenic clone A1 trophozoites overexpressing various ehcp genes. Trophozoites of E. histolytica clone A1 were transfected with episomal plasmids as indicated and selected with G418. Subsequently, lysates (4 µg protein/ml) of the cells were separated on SDS-PAGE gels copolymerized with gelatin. To visualize the CP activity of proteins, gels were stained with Coomassie blue and the picture was inverted. Standards are indicated in kDa on the left.