TABLE 1 .
Expression of ehcp genes in trophozoites of cell line B, clone B2, and clone A1 cultivated under axenic conditions
| ehcp gene | GenBank accession no. | ΔCTa |
||
|---|---|---|---|---|
| Cell line B | Clone B2 | Clone A1 | ||
| ehcp-a1 | XM_645064 | −0.91 ± 0.68 | 0.36 ± 0.74 | 0.20 ± 0.24 |
| ehcp-a2 | XM_645550 | −0.92 ± 0.68 | 0.37 ± 0.52 | 0.58 ± 0.30 |
| ehcp-a3 | XM_648162 | 10.73 ± 1.85 | 12.84 ± 0.45 | 11.25 ± 0.26 |
| ehcp-a4 | XM_651510 | 4.74 ± 0.80 | 5.69 ± 0.58 | 5.01 ± 1.07 |
| ehcp-a5 | XM_645845 | 2.06 ± 0.46 | 2.29 ± 0.70 | 1.84 ± 0.39 |
| ehcp-a6 | XM_652272 | 7.37 ± 0.29 | 7.54 ± 0.92 | 5.18 ± 0.42 |
| ehcp-a7 | XM_643904 | 1.75 ± 0.88 | 2.45 ± 0.81 | 3.36 ± 0.52 |
| ehcp-a8 | XM_652354 | 8.21 ± 1.36 | 8.77 ± 0.33 | 8.5 ± 0.13 |
| ehcp-a9 | XM_650583 | ND | 11.18 ± 0.49 | 10.32 ± 0.17 |
| ehcp-a10 | XM_646055 | 8.27 ± 0.15 | 9.51 ± 0.85 | 8.76 ± 0.22 |
| ehcp-a11 | XM_646598 | 4.67 ± 0.59 | 5.52 ± 0.79 | 4.93 ± 0.04 |
| ehcp-a12 | XM_648731 | 9.66 ± 0.23 | 9.61 ± 0.65 | 8.97 ± 0.11 |
| ehcp-a13 | Not annotated | 6.45 ± 0.30 | 7.44 ± 0.95 | 6.58 ± 0.12 |
| ehcp-b1 | XM_646489 | 14.17 ± 2.44 | 14.38 ± 0.67 | 11.4 ± 0.46 |
| ehcp-b2 | XM_001914414 | ND | 14.56 ± 1.20 | 9.3 ± 0.38 |
| ehcp-b3 | XM_651655 | 10.52 ± 2.10 | 12.53 ± 0.55 | 11.76 ± 0.4 |
| ehcp-b4 | XM_643409 | 5.66 ± 0.30 | 7.17 ± 0.72 | 6.2 ± 0.1 |
| ehcp-b5 | XM_647579 | 10.13 ± 0.29 | 10.44 ± 0.89 | 9.46 ± 0.14 |
| ehcp-b7 | XM_645308 | 8.02 ± 0.84 | 8.86 ± 0.65 | 10.68 ± 0.16 |
| ehcp-b8 | XM_645957 | 18.54 ± 0.52 | 17.65 ± 0.47 | 18.31 ± 0.53 |
| ehcp-b9 | XM_647901 | 10.06 ± 0.06 | 10.37 ± 0.40 | 10.3 ± 0.21 |
| ehcp-b10 | XM_643214 | ND | 12.97 ± 4.09 | 14.95 ± 0.92 |
| ehcp-c1 | XM_649361 | 9.93 ± 0.92 | 9.47 ± 0.61 | 8.3 ± 0.23 |
| ehcp-c2 | XM_651540 | ND | 9.93 ± 2.36 | 6.91 ± 0.57 |
| ehcp-c3 | XM_650036 | ND | 7.82 ± 1.05 | 6.62 ± 0.91 |
| ehcp-c4 | XM_650708 | 4.79 ± 0.87 | 4.63 ± 0.74 | 4.48 ± 0.02 |
| ehcp-c5 | XM_649708 | 4.99 ± 0.47 | 5.98 ± 1.44 | 5.77 ± 0.28 |
| ehcp-c6 | XM_646461 | ND | 12.94 ± 3.3 | 7.5 ± 0.02 |
| ehcp-c9 | XM_649919 | ND | 7.40 ± 0.91 | 5.85 ± 0.02 |
| ehcp-c11 | XM_642991 | ND | 13.94 ± 3.37 | 9.79 ± 0.66 |
| ehcp-c12 | XM_645737 | ND | 10.14 ± 1.31 | 8.22 ± 1.1 |
| ehcp-c13 | XM_651464 | 7.19 ± 0.31 | 7.93 ± 0.54 | 6.16 ± 0.01 |
| ehicp1 | XM_648163 | ND | 0.92 ± 0.06 | 0.89 ± 0.22 |
| ehicp2 | XM_644271 | ND | 2.2 ± 0.09 | 1.89 ± 0.13 |
a
Concentration relative to ehactin, which was used as a normalizer. ND, not determined; ΔCT, threshold cycle. At least two biological replicates were analyzed in duplicate.