TABLE 2 .
Expression of ehcp genes in trophozoites derived from ALAs of gerbils and mice analyzed by qRT-PCR
| ehcp gene | GenBank accession no. | ΔΔCTa |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cell line B (axenic culture) calibrator 7 dpi |
Cell line B gerbil ALA 7 dpib |
Clone B2 (axenic culture) calibrator 7 dpi |
Clone B2 mouse ALA |
|||||||
| 24 hpi |
48 hpi |
72 hpi |
||||||||
| I | II | I | II | I | II | |||||
| ehcp-a1 | XM_645064 | 1 | 2.35 ±0.91 | 1 | 1.53 | 0.84 | 1.08 | 0.55 | 1.69 | 2.67 |
| ehcp-a2 | XM_645550 | 1 | 0.47 ±0.10 | 1 | 0.90 | 0.64 | 0.55 | 1.12 | 0.32 | 0.31 |
| ehcp-a3 | XM_648162 | 1 | 55.59 ±33.42* | 1 | 8.56 | 345.2 | 8.92 | 28.8 | 10.89 | 8.05 |
| ehcp-a4 | XM_651510 | 1 | 16.01 ± 11.94* | 1 | 0.79 | 1.74 | 1.93 | 1.09 | 7.98 | 5.84 |
| ehcp-a5 | XM_645845 | 1 | 1.48 ± 0.99 | 1 | 2.52 | 1.11 | 3.18 | 5.04 | 4.06 | 8.04 |
| ehcp-a6 | XM_652272 | 1 | 2.18 ± 0.32 | 1 | 1.60 | 1.30 | 5.73 | 3.36 | 3.2 | 12.49 |
| ehcp-a7 | XM_643904 | 1 | 2.15 ± 0.52 | 1 | 9.78 | 86.23 | 4.11 | 13.51 | 13.39 | 25.0 |
| ehcp-a8 | XM_652354 | 1 | 1.33 ± 0.47 | 1 | 1.26 | 0.42 | 2.50 | 1.64 | NA | 0.66 |
| ehcp-a9 | XM_650583 | ND | ND | 1 | 1.68 | 1.89 | 2.73 | 2.81 | 2.56 | 1.55 |
| ehcp-a10 | XM_646055 | 1 | 18.45 ± 2.35* | 1 | 2.09 | 20.22 | 5.91 | 12.42 | NA | NA |
| ehcp-a11 | XM_646598 | 1 | 0.61 ± 0.40 | 1 | 0.29 | 0.54 | 1.45 | 1.19 | 1.33 | 0.53 |
| ehcp-a12 | XM_648731 | 1 | 0.83 | 1 | 0.32 | NA | 1.05 | 0.38 | NA | 2.16 |
| ehcp-a13 | Not annotated | 1 | 1.17 ± 0.62 | 1 | 1.07 | NA | 1.4 | 0.77 | NA | NA |
| ehcp-b1 | XM_646489 | 1 | NA | 1 | NA | NA | NA | NA | NA | NA |
| ehcp-b2 | XM_001914414 | ND | ND | 1 | NA | NA | NA | NA | NA | NA |
| ehcp-b3 | XM_651655 | 1 | 2.5 ± 0.33 | 1 | 1.43 | 1.36 | NA | NA | NA | NA |
| ehcp-b4 | XM_643409 | 1 | 0.52 ± 0.33 | 1 | 1.65 | NA | 1.12 | 0.61 | 1.52 | 2.06 |
| ehcp-b5 | XM_647579 | 1 | 1.61 ± 0.83 | 1 | 0.87 | 0.82 | 1.98 | 2.33 | NA | 2.30 |
| ehcp-b7 | XM_645308 | 1 | 0.6 ± 0.42 | 1 | 1.51 | 0.27 | 0.86 | 0.87 | 0.67 | 0.81 |
| ehcp-b8 | XM_645957 | 1 | 116.6 ± 77.37* | 1 | NA | NA | NA | NA | NA | NA |
| ehcp-b9 | XM_647901 | 1 | 11.08 ± 4.0* | 1 | 1.09 | 0.69 | 0.61 | 1.34 | 2.49 | 1.45 |
| ehcp-b10 | XM_643214 | ND | ND | 1 | NA | NA | NA | NA | NA | NA |
| ehcp-c1 | XM_649361 | 1 | 0.8 ± 0.5 | 1 | 0.63 | 0.8 | NA | NA | NA | 2.02 |
| ehcp-c2 | XM_651540 | ND | ND | 1 | NA | NA | NA | NA | NA | NA |
| ehcp-c3 | XM_650036 | ND | ND | 1 | NA | NA | NA | NA | NA | NA |
| ehcp-c4 | XM_650708 | 1 | 2.49 ± 0.95 | 1 | 1.50 | 1.20 | 3.14 | 2.32 | 1.92 | 2.59 |
| ehcp-c5 | XM_649708 | 1 | 0.63 ± 0.32 | 1 | 0.51 | 0.32 | 0.5 | 0.38 | 0.23 | 0.35 |
| ehcp-c6 | XM_646461 | ND | ND | 1 | NA | 2.76 | NA | NA | NA | NA |
| ehcp-c9 | XM_649919 | ND | ND | 1 | 1.27 | 1.14 | 1.28 | 1.33 | NA | 1.42 |
| ehcp-c11 | XM_642991 | ND | ND | 1 | NA | NA | NA | NA | NA | NA |
| ehcp-c12 | XM_645737 | ND | ND | 1 | NA | 1.1 | NA | NA | 2.34 | 1.26 |
| ehcp-c13 | XM_651464 | 1 | 4.25 ± 1.88* | 1 | 2.75 | 1.1 | 2.49 | 2.04 | 3.04 | 2.93 |
a
Relative differences in gene expression; ehactin was used as a normalizer. Bold indicates differentially expressed genes. NA, no amplificate in respective qRT-PCR; ALA, amoebic liver abscess; ND, not determined; I and II indicate two different animals.
b
The threshold for defining genes as being differentially expressed was set at 3.0. The expression of the various ehcp genes of amoebae derived from gerbil livers was analyzed at least two times in duplicate. P values of differentially expressed genes are expressed as follows: *, P < 0.05.