TABLE 3 .
Expression and CP activity of clone A1 transfectants overexpressing various ehcp genes
| Clone A1 transfectant | Relative expression (ΔΔCT method)a | CP activity |
|
|---|---|---|---|
| mU/mg | Fold activity to control (P value) | ||
| pNC | 1 | 0.49 ± 0.47 | 1 |
| pNC-CPA1 | 14.1 ± 13 | 24.3 ± 1.1 | 49.6 (<0.0001)*** |
| pNC-CPA2 | 6.1 ± 1.7 | 69.5 ± 19.4 | 141.8 (0.0012)** |
| pNC-CPA3 | 22.20 ± 20.35 | 1.2 ± 0.38 | 2.4 (NS) |
| pNC-CPA4 | 387.5 ± 94.5 | 7.7 ± 2.9 | 15.7 (0.0007)*** |
| pNC-CPA5 | 3.16 ± 2.01 | 30.6 ± 18.3 | 62.4 (0.0011)** |
| pNC-CPA6 | 119.2 ± 21.7 | 0.9 ± 0.6 | 1.8 (NS) |
| pNC-CPA7 | 7 ± 4.4 | 5.2 ± 4.5 | 10.6 (0.0080)** |
| pNC-CPA10 | 642.4 ± 54.1 | 2.9 ± 2.4 | 5.9(0.0043)** |
| pNC-CPB8 | 9,370 ± 13,028 | 3.6 ± 2.9 | 7.3(0.043)* |
| pNC-CPB9 | 589 ± 1,097 | 3.8 ± 3.6 | 7.8(0.0173)* |
| pNC-CPC13 | 19.83 ± 12.17 | 2.1 ± 0.9 | 4.3(0.0043)** |
a
Relative expression of the ehcp gene that should be overexpressed in the respective transfectant was analyzed. Ehactin was used as a normalizer. NS, not significant; CP, cysteine peptidases.