Table 1.
Tracked NPC1 vesicle movements around inclusions in A. phagocytophilum (with and without oxytetracycline treatment) and C. trachomatis-infected RF/6A cellsa
| Movement of NPC1 vesicles associated with inclusions |
Ap/CTL (n=98) |
Ap/OTC (n=133) |
Ct (n=100) |
|---|---|---|---|
| Average maximum speed (µm/s) | 0.097±0.034 | 0.051±0.017** | 0.046±0.026** |
| Average speed (µm/s) | 0.046±0.036 | 0.020±0.021** | 0.025±0.016** |
a
RF/6A cells were transfected with NPC1-YFP plasmid and infected with mCherry-A. phagocytophilum with or without oxytetracycline (OTC) treatment for 10 h or C. trachomatis. Inclusion-bound or -associated vesicles were tracked for 5 min. Data are expressed as mean ± standard deviation (n=98–133). Ap, A. phagocytophilum. Ct, C. trachomatis
**
P < 0.01 (unpaired two-tailed t-test).