Figure 6.

3′ rare codon clustering is not a general property of tear proteins. (A) RCC analysis of LCN1 coding sequence with the human codon frequency table. (B) The least %Min for each the first 21 tear proteins of the human tear proteome (see Table 1 in Ref. 1) was plotted as a fraction of coding sequence length (closed circles). Included was the least %Min for LCN1 (open square), and each of seven LACRT orthologs (#1, human; #2, chimp; #3, mouse lemur; #4, cat; #5, tree shrew; #6, shrew; #7, horse). Each was analyzed by the RCC, respectively, using human, chimp, mouse lemur, cat, tree shrew, shrew, and horse codon frequency tables. (A) and (B) were calculated with coding sequence for the signal peptide included.