Skip to main content
. 2012 Aug 24;5(2):99–110. doi: 10.1093/jmcb/mjs052

Figure 1.

Figure 1

Eif4g3 gene expression is down-regulated in the PLNs of Deaf1-KO mice. (A) Venn diagram showing the number of genes down-regulated by ≥2-fold in the PLNs of 4, 12, and 30-week old Deaf1-KO mice vs. age-matched controls (4-week old, n = 2 per group; 12 and 30-week old, n = 3 mice per group), as measured by two-color microarray analysis. Multiple probes for the same gene and unknown genes have been omitted from the regions of overlap. (B) Heatmap image showing changes in Eif4g3, Ercc4, and Ncr1 gene expression in individual Deaf1-KO mice compared with age-matched WT controls. qPCR data showing reduced expression of Eif4g3 (C) and no change in expression of Eif4g1 (D) in the PLNs of Deaf1-KO mice compared with littermate controls (n ≥ 6 mice per group, two-tailed Mann–Whitney test). Eif4g1 encodes for eIF4GI, a homolog of eIF4GII. (E) A schematic diagram showing eIF4GI or eIFGII in the 48S translation pre-initiation complex. eIF4GI and eIF4GII (encoded by Eif4g3) can act as the scaffold for the poly (A)-binding protein (PABP) and other members of the eIF family (shown in blue). The 5′ cap and poly-A tail of the mRNA strand (green) binds to eIF4E and PABP, respectively.