Figure 2.

RyR1 in β-sarcoglycan deficient muscle is cysteine-nitrosylated, oxidized, and depleted of calstabin1. (A) Representative immunoblot of immunoprecipitated RyR1 from wild-type (WT) and β-sarcoglycan deficient (Sgcb−/−) EDL muscles. Antibodies against RyR1-S2844 phosphorylation (P*RyR1), cysteine-nitrosylated (Cys NO) proteins, calstabin1, and the protein oxidation marker 2,4- dinitrophenylhydrazone (DNP) was used. The muscle from a mouse treated with S107 is marked (+). (B) Bar graph showing average band intensities normalized to RyR1 expression (mean ± SEM, n = 3 for all groups).