Table 2. Different apo ERK2 crystals and ERK2–docking peptide complexes.
The binding affinity of peptides to ERK2 are from Garai et al. (2012 ▶); amino acids in consensus sequence positions are shown in bold.
| Unit-cell parameters | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Construct | Peptide | a (Å) | b (Å) | c (Å) | α (°) | β (°) | γ (°) | Space group | Resolution (Å) | Peptide sequence | Binding affinity (µM) |
| ERK2 WT | Apo | 44.9 | 65.3 | 116.7 | 90.0 | 90.0 | 90.0 | P212121 | 1.55 | — | — |
| ERK2_AA | Apo | 44.7 | 71.5 | 121.1 | 90.0 | 90.0 | 90.0 | P212121 | 1.90 | — | — |
| ERK2_AAG | Apo | 86.5 | 86.5 | 311.1 | 90.0 | 90.0 | 120.0 | H32 | 2.50 | — | — |
| ERK2 WT† | MNK1 | 65.4 | 65.9 | 95.0 | 90.0 | 90.0 | 90.0 | P212121 | 1.55 | MKLSPPSKSRLAQRRALA | 0.7 |
| ERK2_AA† | RSK1 | 41.7 | 59.0 | 155.7 | 90.0 | 90.0 | 90.0 | P212121 | 2.40 | PQLKPIESSILAQRRVRKLSPTTL | 0.3 |
| ERK2_AA | RSK1_SQAA | 41.5 | 58.8 | 79.2 | 100.9 | 90.0 | 90.0 | P1 | 2.30 | PQLKPIEASILAA RRVRKLSPTTL | 2 |
| ERK2_AAGS | MKK2 | 41.8 | 58.5 | 159.2 | 90.0 | 90.0 | 90.0 | P212121 | 2.20 | RRKPVLPALTINP | 8 |
†
These ERK2–docking peptide complexes were reported in Garai et al. (2012 ▶).