Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cancer Res. 2012 Mar 15;72(9):2251–2261. doi: 10.1158/0008-5472.CAN-11-3386

Figure 1. The p53 and Rb pathways are dispensable for OPN upregulation in senescence.

Figure 1

A. Western Blot analysis of BJ fibroblasts stably transduced with a p53 mutant (DD) or CDK4/cyclinD1 (DK) fusion construct that inhibits wildtype p53 and Rb, respectively. β- and γ-actin serve as loading controls for the two cell lines. B. SA-βgal staining of BJ fibroblasts treated with vehicle alone (vehicle) or bleomycin (bleo) and BJ fibroblasts stably transduced with DD or DK treated with bleomycin (DD+Bleo and DK+Bleo, respectively). Scale bar is 100 microns. C. OPN mRNA levels were measured by quantitative PCR (qPCR) in cells described in A following treatment with vehicle or bleomycin. Vehicle-treated cells are defined as 1. The mean ± STDEV is shown (n=3).