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. 2013 Mar 21;9(3):e1003397. doi: 10.1371/journal.pgen.1003397

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© 2013 Sarshad et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic representation of V5-tagged wt and mutated NM1 constructs stably expressed in HEK293T cell lines. (B) Steady state expressions of V5-tagged wt and mutated NM1 constructs were monitored on immunoblots for V5 and actin antibodies as loading control. (C) Co-precipitations of SNF2h and PCAF from total lysates obtained from HEK293T cells stably expressing wt and mutated V5-tagged NM1 constructs as indicated. 10% of the input is shown in Lane 1. IP, immunoprecipitation. (D) Semiquantitative densitometric analysis of endogenous SNF2h levels co-precipitated with V5-tagged NM1 constructs relative to input.