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. 2013 Feb 21;10:21. doi: 10.1186/1742-4690-10-21

Table 2.

IN mutations of NL-ADA or NL-IN-D64A-ADA viruses in Figure5B and5C

Virus Clone # RNA Amino acid
WT/RAL
 #1
 -
 -
 
 #5
 -
 -
 
 #6
 -
 -
 
 #7
 -
 -
 
 #8
 -
 -
 
 #9
 -
 -
 
 #10
 -
 -
 
 #2
 A144G
 E48E (Silent)
 
 #3
 A87G
 P29P (Silent)
 
 #4
 A731G
 R244K
D64A/DMSO
 #1
 A191C
 D64A
 
 #2
 A191C
 D64A
 
 #3
 A191C
 D64A
 
 #4
 A191C
 D64A
 
 #7
 A191C
 D64A
 
 #9
 A191C
 D64A
 
 #5
 A191C, A772G
 D64A, K258E
 
 #6
 A191C, U842A
 D64A, V281E
 
 #8
 A38G, A191C, G462A
 E13G, D64A, M154I
 
 #10
 A191C, A279, A809G
 D64A, T93T (Silent), D270G
D64A/RAL
 #1
 A191C
 D64A
 
 #4
 A191C
 D64A
 
 #8
 A191C
 D64A
 
 #10
 A191C
 D64A
 
 #2
 A191C, G806C
 D64A, R269T
 
 #3
 A191C, A718G
 D64A, K240E
 
 #5
 A191C, G829U
 D64A, G277C
 
 #6
 A191C, U598C
 D54A, I200T
 
 #9
 A191C, G462A
 D64A, M154I
  #7 G7A, G39A, G103A, A191C D3N, E13E (Silent), E35K, D64A

Nucleotide sequence of progeny viral RNA was analyzed. In two of 20 clones examined, we identified M1541, which was reported as a mutation related to RAL-resistant phenotype [30].