Skip to main content
. 2013 Mar 21;9(3):e1003231. doi: 10.1371/journal.ppat.1003231

Figure 8. EV71 2Apro cleaves MAVS at multiple residues.

Figure 8

(A) Schematic diagram of the Protease-Glo assay of 2Apro on MAVS extra-membrane region. (B) The first-round screening of 86 constructs containing the coding region for the 12-mer polypeptides covering the MAVS extra-membrane region. Luciferase assay results are shown together with gel analysis results. (C) The second-round screening of the 10 positive constructs selected in the first round of screening (Table 1) using a mutated pGlosensor-10F vector containing a mutation from Gly to Ala in the linker region. (D) Protease-Glo assay of M209, M251, and M265. These constructs were mutated from the 3 selected constructs from the second round of screening (Table 2), and each contains a point mutation (from Gly to Ala) at residue 209, 251, or 265. (E) In vitro cleavage assay of EV71 2Apro on in vitro translated extra-membrane region of MAVS (MAVS-EM) and its mutants (MAVS-EM-3M) labeled with FluoroTect GreenLys by gel analysis.