Table 2. Relative mRNA expression levels of Zn transporters of the ZnT and ZIP class as well as DMT-1 in various cells.
| |
Mouse islets |
MIN6 cells |
3T3 cells |
|---|---|---|---|
| Mean ± SEM | Mean ± SEM | Mean ± SEM | |
|
ZIP1 |
8.40 × 10-02 ± 1.07 × 10-02 |
1.47 × 10-02 ± 6.83 × 10-04 |
6.73 × 10-02 ± 3.57 × 10-03 |
|
ZIP2 |
4.94 × 10-05 ± 1.27 × 10-05 |
3.72 × 10-05 ± 1.26 × 10-05 |
1.27 × 10-05 ± 1.37 × 10-06 |
|
ZIP3 |
4.83 × 10-03 ± 2.61 × 10-04 |
2.94 × 10-03 ± 5.42 × 10-04 |
2.02 × 10-03 ± 2.14 × 10-04 |
|
ZIP4 |
7.05 × 10-04 ± 3.31 × 10-04 |
2.21 × 10-05 ± 2.21 × 10-05 |
1.50 × 10-05 ± 1.05 × 10-06 |
|
ZIP5 |
5.14 × 10-04 ± 1.52 × 10-04 |
1.29 × 10-05 ± 1.29 × 10-05 |
1.77 × 10-07 ± 5.74 × 10-08 |
|
ZIP6 |
9.84 × 10-04 ± 1.76 × 10-04 |
1.07 × 10-03 ± 2.27 × 10-04 |
2.76 × 10-04 ± 1.92 × 10-05 |
|
ZIP7 |
2.13 × 10-01 ± 4.76 × 10-02 |
1.04 × 10-01 ± 1.31 × 10-02 |
4.81 × 10-02 ± 1.80 × 10-03 |
|
ZIP8 |
1.33 × 10-03 ± 2.30 × 10-04 |
1.95 × 10-02 ± 3.06 × 10-03 |
7.15 × 10-03 ± 3.45 × 10-04 |
|
ZIP9 |
4.36 × 10-03 ± 1.40 × 10-03 |
3.78 × 10-03 ± 6.17 × 10-04 |
4.21 × 10-03 ± 2.39 × 10-04 |
|
ZIP10 |
5.64 × 10-04 ± 1.37 × 10-04 |
1.28 × 10-03 ± 1.37 × 10-04 |
4.29 × 10-03 ± 1.17 × 10-04 |
|
ZIP11 |
3.77 × 10-03 ± 1.37 × 10-03 |
2.89 × 10-04 ± 2.90 × 10-06 |
1.49 × 10-03 ± 1.15 × 10-04 |
|
ZIP12 |
3.33 × 10-08 ± 3.33 × 10-08 |
3.33 × 10-08 ± 3.33 × 10-08 |
1.14 × 10-07 ± 4.14 × 10-08 |
|
ZIP13 |
8.59 × 10-03 ± 2.68 × 10-03 |
3.56 × 10-03 ± 4.33 × 10-04 |
6.21 × 10-03 ± 6.88 × 10-04 |
|
ZIP14 |
2.35 × 10-03 ± 2.58 × 10-05 |
1.49 × 10-03 ± 2.81 × 10-04 |
3.01 × 10-03 ± 1.71 × 10-04 |
|
ZnT1 |
4.38 × 10-03 ± 1.71 × 10-03 |
2.79 × 10-03 ± 4.10 × 10-04 |
1.34 × 10-03 ± 7.04 × 10-05 |
|
ZnT2 |
4.88 × 10-04 ± 7.66 × 10-05 |
1.29 × 10-05 ± 1.08 × 10-05 |
2.34 × 10-06 ± 1.92 × 10-06 |
|
ZnT3 |
1.82 × 10-05 ± 1.41 × 10-05 |
5.68 × 10-06 ± 3.17 × 10-06 |
6.95 × 10-08 ± 8.89 × 10-09 |
|
ZnT4 |
1.75 × 10-03 ± 1.34 × 10-04 |
2.27 × 10-03 ± 1.14 × 10-04 |
1.68 × 10-02 ± 5.13 × 10-04 |
|
ZnT5 |
1.37 × 10-02 ± 2.28 × 10-03 |
2.60 × 10-02 ± 3.27 × 10-03 |
7.75 × 10-03 ± 3.61 × 10-04 |
|
ZnT6 |
5.64 × 10-03 ± 8.13 × 10-04 |
5.20 × 10-03 ± 7.52 × 10-04 |
9.87 × 10-03 ± 3.96 × 10-04 |
|
ZnT7 |
1.01 × 10-02± 5.94 × 10-04 |
5.82 × 10-03 ± 8.71 × 10-04 |
1.62 × 10-02 ± 2.79 × 10-04 |
|
ZnT8 |
5.76 × 10-02± 2.33 × 10-02 |
1.16 × 10-01 ± 1.89 × 10-02 |
0.00 ± 0.00 |
|
ZnT9 |
2.59 × 10-03 ± 6.62 × 10-04 |
2.86 × 10-03 ± 2.01 × 10-04 |
8.00 × 10-03 ± 6.33 × 10-05 |
|
ZnT10 |
0.00 ± 0.00 |
0.00 ± 0.00 |
4.52 × 10-07 ± 3.06 × 10-07 |
| DMT-1 | 2.56 × 10-02 ± 6.98 × 10-03 | 3.38 × 10-02 ± 1.90 × 10-02 | 9.56 × 10-03 ± 9.22 X 10-05 |
mRNA levels of Zn transporters of the ZnT and ZIP families as well as DMT-1 in primary mouse islets, MIN6 cells and 3T3 relative to the house keeping genes β actin and 18S (n = 3).