A) Representative p16 immunohistochemistry.
B) High p16 staining fails to stratify women with DCIS that develop subsequent disease. Recurrence-free survival plots demonstrate that women with DCIS staining high or low for p16 develop subsequent disease at the same rate.
C) Ki67 index labelling stratifies recurrence-free survival in women with DCIS.
D) DCIS exhibiting high p16 immunostaining and elevated Ki67 identifies women that have a reduced recurrence-free survival.
E) Ki67 does not differentiate risk in DCIS lesions with low p16. Box plots and corresponding p-values were determined using Wilcoxon/Kruskal-Wallis rank of sums test. Survival plots were generated using Kaplan-Meyer analysis.