Table 1. Standard curve equations, reaction efficiencies and starting DNA concentrations of eight Batrachochytrium dendrobatidis strains.
| Strain | Origin | DNAConcentration(ng/µL) ± SD | Standard Set A:Zoospore Counts (R2) | Efficiency(10(−1/slope)–1×100%) | Standard Set B: DNAdilutions (R2) | Efficiency (%) |
| JEL404 * | USA (Maine) | 0.33±0.01 | y = −3.13x +31.6 (0.990) | 109% | y = −3.42x +14.3 (0.999) | 96.1% |
| MexMkt | Mexico | 1.01±0.04 | y = −3.31x +33.9 (0.999) | 101% | y = −3.48x +15.9 (0.996) | 93.8% |
| JEL427 | Puerto Rico | 2.76±0.06 | y = −3.15x +30.8 (0.998) | 108% | y = −3.44x +14.5 (0.999) | 95.3% |
| PAB01 * | Puerto Rico | 0.65±0.04 | y = −3.05x +30.8 (0.992) | 113% | y = −3.28x +16.3 (0.997) | 102% |
| LFT001_01 | Brazil | 4.11±0.10 | y = −3.36x +30.5 (0.999) | 98.4% | y = −3.48x +14.3 (0.999) | 93.8% |
| CLFT023 | Brazil | 2.09±0.05 | y = −3.35x +30.4 (0.993) | 98.8% | y = −3.36x +13.2 (0.999) | 98.4% |
| CLFT024 * | Brazil | 4.64±0.03 | y = −3.35x +30.5 (0.993) | 98.8% | y = −3.52x +15.1 (0.999) | 92.3% |
| LBAbercrom | Australia | 1.34±0.05 | y = −3.22x +30.4 (0.997) | 104% | y = −3.55x +12.6 (0.999) | 91.3% |
Standard curve coefficients (slopes and intercepts) were estimated from the linear regression equation between cycle threshold (Ct) and log-transformed zoospore counts. Average (± SD) DNA concentration of extracts is based on 107 zoospores in 200 µL extraction buffer.
*
106 zoospores.