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. 2013 Mar 21;9(3):e1003368. doi: 10.1371/journal.pgen.1003368

Figure 2. Microarray analysis in HDFs reveal that MALAT1-depletion reduces the expression of genes involved in cell cycle progression.

Figure 2

(A) Top significant biological processes for genes whose transcript levels are reduced (Z ratio ≥2.5) in MALAT1-depleted fibroblasts. Cell cycle is the most affected biofunction in MALAT1-depleted cells. (B–D) qRT-PCR analyses in control (scr) versus MALAT1-antisense (AS1 & AS2) oligo-treated HDFs reveal decreased mRNA levels of genes involved in G1/S transition (B), mitotic progression (C), and other general cellular processes (D) upon MALAT1 depletion. (E) The expression of indicated genes is determined by immunoblotting using extracts from control and MALAT1-depleted WI-38 cells. MEK2 is used as loading control.