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. 2013 Mar 21;7(3):e2121. doi: 10.1371/journal.pntd.0002121

Figure 4. Expression of a Fam50 gene (Tb927.7.380) in T. brucei.

Figure 4

A. Western blot; 2×107 cells were sampled from either induced (+) or uninduced (−) cells after 1-day induction with 1 µg/mL tetracycline. Markers shown in kDa. B. Immunofluorescence analysis of Tb927.7.380 expression in T. brucei, showing co-staining of non-permeabilised cells with plasma membrane protein VSG 221 (top) and PFR2 (bottom). Left: Merged images with color combination for DAPI-stain of the nucleus and kinetoplast (blue), fluorescent stain of HA epitope tag (red) and VSG/PFR fluorescence (green). Right: merged pictures from phase and fluorescence. Inset: co-staining of non-permeabilised and permeabilised cells with Rab11 (intracellular marker). No fluorescence was seen from non-transfected cells (data not shown). GFP expressed from pDEX-577 vector localised to the cytoplasm (data not shown). Scale bar is 2 µm.