Table 1.
N. furzeri transcriptome libraries and sequencing methods
Library | Specimens used for RNA-isolation | Sequencing technology | Number of reads | Average length (bp) | Total length (Mb) |
---|---|---|---|---|---|
1 |
one male GRZ, 9 weeks, whole body; normaliseda |
Sanger |
131,808 |
734 |
97 |
2 |
one male GRZ, 10 weeks, whole body; normaliseda |
454/Roche GS-FLX |
751,885 |
223 |
168 |
3 |
one male GRZ, 10 weeks, whole body |
454/Roche GS-FLX |
415,426 |
225 |
94 |
4 |
six male and female GRZ, 8 weeks, whole body; normalisedb |
454/Roche GS-Titanium |
2,351,236 |
369 |
869 |
5 |
three GRZ, 1 week, whole body |
Solexa/Illumina, 2 x 150 bp |
89,730,910 |
151 |
6,774 |
6 |
five male GRZ, 5 weeks, skin |
Solexa/Illumina, 2 x 101 bp |
55,410,042 |
101 |
5,596 |
|
|
Solexa/Illumina, 76 bp |
33,947,044 |
76 |
2,579 |
7 |
five male GRZ, 5 weeks, brain |
Solexa/Illumina, 2 x 101 bp |
60,997,820 |
101 |
6,161 |
|
|
Solexa/Illumina, 76 bp |
36,117,265 |
76 |
2,744 |
8 |
three male GRZ, 14 weeks, skin |
Solexa/Illumina, 76 bp |
35,963,921 |
76 |
2,733 |
9 |
three male GRZ, 14 weeks, brain |
Solexa/Illumina, 76 bp |
27,369,310 |
76 |
2,080 |
10 |
four male MZM-0403, 5 weeks, skin |
Solexa/Illumina, 76 bp |
31,727,935 |
76 |
2,411 |
11 |
five male MZM-0403, 5 weeks, brain |
Solexa/Illumina, 76 bp |
34,720,289 |
76 |
2,638 |
12 |
five male MZM-0403, 31 weeks, skin |
Solexa/Illumina, 76 bp |
30,268,649 |
76 |
2,300 |
13 |
three male MZM-0403, 31 weeks, brain |
Solexa/Illumina, 76 bp |
31,423,327 |
76 |
2,388 |
467,676,512 | 39,632 |
aNormalisation was done by Evrogen.
bNormalisation was done by Vertis.