Changes in connexin mRNA in the OB across dark and light phases of the diurnal cycle. Line plots of (A) connexin36 (Cx36), (B) connexin43 (Cx43), and (C) connexin45 (Cx45) mRNA expression over 48-h. Sample size and notation as in Fig. 1. Cx36, Cx43, and Cx45 mRNA changes were statistically significant across the 48 h (Kruskal–Wallis H test, P < 0.05). Cx36 was rhythmic for the first 24-h tested and both Cx43 and Cx45 were rhythmic for the second 24-h tested (harmonic regression analysis, P < 0.05). Cx36 and Cx43 mRNAs were statistically significant only for the first 24-h period as tested by JTK_CYCLE analysis (P < 0.05), while Cx45 was not significant (P > 0.05).